quantitative polymerase chain reaction (qpcr) Search Results


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tiangen biotech co reverse transcription-fluorescence quantitative polymerase chain reaction (qpcr) kit
Prediction of the miRNA-584-5p target. A: Potential binding sites of miRNA-584-5p and the 3’-untranslated region of RUNX family transcription factor 2 (RUNX2); B: Dual luciferase reporter gene assay; C: Quantitative <t>polymerase</t> chain reaction determination of RUNX2 expression; D: Western blot determination of RUNX2 protein expression. One-way ANOVA and t tests were used to demonstrate differences in RUNX2 responses between the miR584-5p mimic and negative control groups. a P < 0.05; b P < 0.01. RUNX2: RUNX family transcription factor 2; NC: Negative control; wt: Wild type; mut: Mutant; miRNA: MicroRNA.
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Beijing TransGen Biotech quantitative polymerase chain reaction (qpcr) kit
Prediction of the miRNA-584-5p target. A: Potential binding sites of miRNA-584-5p and the 3’-untranslated region of RUNX family transcription factor 2 (RUNX2); B: Dual luciferase reporter gene assay; C: Quantitative <t>polymerase</t> chain reaction determination of RUNX2 expression; D: Western blot determination of RUNX2 protein expression. One-way ANOVA and t tests were used to demonstrate differences in RUNX2 responses between the miR584-5p mimic and negative control groups. a P < 0.05; b P < 0.01. RUNX2: RUNX family transcription factor 2; NC: Negative control; wt: Wild type; mut: Mutant; miRNA: MicroRNA.
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Prediction of the miRNA-584-5p target. A: Potential binding sites of miRNA-584-5p and the 3’-untranslated region of RUNX family transcription factor 2 (RUNX2); B: Dual luciferase reporter gene assay; C: Quantitative <t>polymerase</t> chain reaction determination of RUNX2 expression; D: Western blot determination of RUNX2 protein expression. One-way ANOVA and t tests were used to demonstrate differences in RUNX2 responses between the miR584-5p mimic and negative control groups. a P < 0.05; b P < 0.01. RUNX2: RUNX family transcription factor 2; NC: Negative control; wt: Wild type; mut: Mutant; miRNA: MicroRNA.
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Qiagen cardiotoxicity quantitative polymerase chain reaction (qpcr) array
Prediction of the miRNA-584-5p target. A: Potential binding sites of miRNA-584-5p and the 3’-untranslated region of RUNX family transcription factor 2 (RUNX2); B: Dual luciferase reporter gene assay; C: Quantitative <t>polymerase</t> chain reaction determination of RUNX2 expression; D: Western blot determination of RUNX2 protein expression. One-way ANOVA and t tests were used to demonstrate differences in RUNX2 responses between the miR584-5p mimic and negative control groups. a P < 0.05; b P < 0.01. RUNX2: RUNX family transcription factor 2; NC: Negative control; wt: Wild type; mut: Mutant; miRNA: MicroRNA.
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tiangen biotech co real-time quantitative polymerase chain reaction (qpcr)-related kits
Prediction of the miRNA-584-5p target. A: Potential binding sites of miRNA-584-5p and the 3’-untranslated region of RUNX family transcription factor 2 (RUNX2); B: Dual luciferase reporter gene assay; C: Quantitative <t>polymerase</t> chain reaction determination of RUNX2 expression; D: Western blot determination of RUNX2 protein expression. One-way ANOVA and t tests were used to demonstrate differences in RUNX2 responses between the miR584-5p mimic and negative control groups. a P < 0.05; b P < 0.01. RUNX2: RUNX family transcription factor 2; NC: Negative control; wt: Wild type; mut: Mutant; miRNA: MicroRNA.
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Lonza human apoptosis 96 stell-aray quantitative real-time polymerase chain (qpcr) array
Prediction of the miRNA-584-5p target. A: Potential binding sites of miRNA-584-5p and the 3’-untranslated region of RUNX family transcription factor 2 (RUNX2); B: Dual luciferase reporter gene assay; C: Quantitative <t>polymerase</t> chain reaction determination of RUNX2 expression; D: Western blot determination of RUNX2 protein expression. One-way ANOVA and t tests were used to demonstrate differences in RUNX2 responses between the miR584-5p mimic and negative control groups. a P < 0.05; b P < 0.01. RUNX2: RUNX family transcription factor 2; NC: Negative control; wt: Wild type; mut: Mutant; miRNA: MicroRNA.
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Seegene Technologies real time reverse transcription-polymerase chain reaction (rt-pcr) for sars-cov-2
Prediction of the miRNA-584-5p target. A: Potential binding sites of miRNA-584-5p and the 3’-untranslated region of RUNX family transcription factor 2 (RUNX2); B: Dual luciferase reporter gene assay; C: Quantitative <t>polymerase</t> chain reaction determination of RUNX2 expression; D: Western blot determination of RUNX2 protein expression. One-way ANOVA and t tests were used to demonstrate differences in RUNX2 responses between the miR584-5p mimic and negative control groups. a P < 0.05; b P < 0.01. RUNX2: RUNX family transcription factor 2; NC: Negative control; wt: Wild type; mut: Mutant; miRNA: MicroRNA.
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Image Search Results


Prediction of the miRNA-584-5p target. A: Potential binding sites of miRNA-584-5p and the 3’-untranslated region of RUNX family transcription factor 2 (RUNX2); B: Dual luciferase reporter gene assay; C: Quantitative polymerase chain reaction determination of RUNX2 expression; D: Western blot determination of RUNX2 protein expression. One-way ANOVA and t tests were used to demonstrate differences in RUNX2 responses between the miR584-5p mimic and negative control groups. a P < 0.05; b P < 0.01. RUNX2: RUNX family transcription factor 2; NC: Negative control; wt: Wild type; mut: Mutant; miRNA: MicroRNA.

Journal: World Journal of Stem Cells

Article Title: MicroRNA-584-5p/RUNX family transcription factor 2 axis mediates hypoxia-induced osteogenic differentiation of periosteal stem cells

doi: 10.4252/wjsc.v15.i10.979

Figure Lengend Snippet: Prediction of the miRNA-584-5p target. A: Potential binding sites of miRNA-584-5p and the 3’-untranslated region of RUNX family transcription factor 2 (RUNX2); B: Dual luciferase reporter gene assay; C: Quantitative polymerase chain reaction determination of RUNX2 expression; D: Western blot determination of RUNX2 protein expression. One-way ANOVA and t tests were used to demonstrate differences in RUNX2 responses between the miR584-5p mimic and negative control groups. a P < 0.05; b P < 0.01. RUNX2: RUNX family transcription factor 2; NC: Negative control; wt: Wild type; mut: Mutant; miRNA: MicroRNA.

Article Snippet: RNA was reverse-transcribed into cDNA using a reverse transcription-fluorescence quantitative polymerase chain reaction (qPCR) kit (Tiangen, Beijing, China) and PCR apparatus (Applied Biosystems, United States) for real-time fluorescence quantification (qPCR) after determining the purity of the RNA samples.

Techniques: Binding Assay, Luciferase, Reporter Gene Assay, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Negative Control, Mutagenesis